Fig. 6.1
Complete laparoscopic column
Endoscopes
The endoscope is the instrument that has the delicate duty of extending the human eye into anatomical cavity and collecting the images to guide the surgery.
Surgical endoscopes utilize high performance optical technology. The choice of endoscope requires a good deal of consideration since it is one of the most expensive pieces of surgical equipment both in terms of capital cost and maintenance. A good endoscope carries out three tasks. It must be able to transmit a good level of brightness, both in bringing light into the operative field and carrying the image to the camera. It must have large depth of field and minimal distortion of image viewed. Finally, the endoscope should also be able to withstand the mechanical and thermal constraints of the sterilization processes.
Structure and Function
The rigid endoscope is constituted by a metal tube that contains two channels. One carries light to illuminate the operative field and the other carries the image of the operative field to the camera. The image channel is made up of a series of lens that are successively placed one after the other and separated by spacers. As the image passes through each lens it is inverted and the number of lenses depends on the length of the endoscope. This system is known as the Inverting Real Image Lens System (IRILS) and permits refraction of the light while maintaining overall luminosity of the endoscope. The “eyepiece” lens at the end of the series acts to magnify the image before it reaches the camera.
So the endoscope can be divided into five distinct parts: (1) objective lens system, (2) rod-lens assembly, (3) ocular lens, (4) light post, and (5) light channel.
Objective Lens System
The lens located at the distal end of the endoscope captures the image and directs it back through the endoscope. Its focal length is generally fixed. It determines the magnification of the object and the field of view of the endoscope. By changing the angle between the long axis of the lens and the axis of the endoscope the direction or “angle of view” can be changed.
Rod-Lens Assembly
A series of spaced glass rod lenses continually refocus the image up through the scope. Metal spacers separate each rod lens to maintain the appropriate focal distance between lenses. The number of lenses depends on the length of the endoscope. A longer scope requires more lenses but this reduces overall luminosity and increases the fragility of the system. In order to minimize the reduction in luminosity due to reflective loss all of the endoscope’s lenses are treated with an anti-glare system. This consists in coating each lens surface with a thin layer of magnesium fluoride, using a vacuum pack process. Thus, it is important to avoid damaging the optical surface of the endoscope.
Ocular Lens
The last lens in the series, at the proximal end of the camera, magnifies and focuses the image for transmits to the camera. This part of the endoscope is designed to allow coupling to the camera lens.
Light Post (Light Guide Connection)
Set at an angle to the main axis of the scope is a connection point for the cold light source cable. This connection must be of high optical and mechanical quality in order to avoid loss of light and overheating.
Light Channel
This runs along the length of the endoscope carrying light from the light post to the tip of the scope. The light is carried in a bundle of glass fibers. At each end of the channel the fibers are cut, polished, and capped to produce a light-transmitting surface.
Endoscope: Function
The optical quality of the endoscope is determined by the same optical laws that govern optical quality in photography.
Focal Length
The focal length in an optical device is the measure of the lens focus. In particular, it coincides with the distance (in mm) between the lenses and the focal plane when the object to be focused is at infinity (so the optical beams are parallel to the axis of the system). The focal length of the endoscope is determined by the aperture of lens at the tip of the laparoscope (objective lenses). An object at the focal distance appears in its natural size within the image. However, when the object is brought closer to the lens, then it appears magnified whereas if it is moved further away then it appears smaller between the system and the object. If the endoscope is coupled to a camera fitted with a mechanical zoom the focal length of the camera can be adjusted. By moving the Charge-Coupled-Device (CCD) sensor away from the eyepiece lens the image is magnified but the field of view is reduced. However, most camera zooms are digital, which means that the image processor in the camera control unit simply magnifies each pixel. Thus although the image may appear magnified the resolution is diminished, leading to a decrease in the quality of the image, which becomes more apparent the more the digital zoom is used.
Brightness
The brightness of source or of a surface is the perception of the amount of light produced by these sources and reflected from that surface. The brightness of the image diminishes further the light that has to travel and the narrows the channel along which it has to pass. Thus a long, small caliber endoscope will transmit an image of much reduced brightness compared to a short, wide caliber scope.
Depth of Field
The depth of field is the distance between near and far objects in the field, which appear focused. For a particular laparoscope this is fixed being determined by the configuration of the lenses and the caliber of the scope with a narrower scope having a deeper depth of field.
Peripheral Light Loss (Vignetting)
There are two reasons why the periphery of the field of view can appear dark. The first is simply that the shape of the image transmitted to the camera by the endoscope is not exactly the same shape as the CCD. This problem can be corrected by image processing that can crop the image. The second cause is due to reduced light transmission through the periphery of the lens. The poorer the quality of the lens the more exaggerated this effect becomes.
Width of Field
The angle of view is the angle subtended by two points diametrically opposite to each other at the extremity of the field of view and the tip of the endoscope. By changing the curvature of the objective lens the angle of view can be changed. However, increasing the curvature of the lens produces more distortion in the peripheral part of the field of view (barrelling or fish-eye effect). Again higher the quality of the lens, the less the distortion.
The desirable features of brightness and depth of field, and angle of view and minimal distortion, require opposite characteristics from the endoscope. This makes it difficult and costly to construct endoscopes with a large depth and width of field, high brightness, and minimal distortion.
Choice of Endoscopes
Most endoscopes used in laparoscopic and endoscopic surgery are monocular endoscopes. A wide choice is available on the market. The choice of the endoscope depends on the specific features of the instrument and the type of surgery to be performed. The characteristics of endoscopes are defined by a set of parameters to understand the type of performance that can support.
Direction (Angle) of View
Endoscopes with varying tip angulations ranging from 0° (standard forward looking scope) to 120° are available.
When laparoscopy was first introduced for digestive procedures, endoscopes with angulated tips of 25, 30, or 40° were widely used. Currently, their use is in decline as the brightness and field of view offered by angled endoscopes is generally inferior to those offered by 0° endoscopes. However, recent angled endoscopes with much improved optical properties are just being introduced. It should be noted that the view provided by an angled endoscope could impair surgical performance particularly in surgeons with limited experience.
Most laparoscopic surgical procedures can be performed with straight (0°) endoscopes. Some surgeons, however, prefer to use endoscopes with an angled line of sight, usually 30°, in order to explore areas that are difficult to visualize (gastroesophageal reflux disease surgery, urological surgery, gynecological surgery).
For the beginner who wants to approach the video-assisted breast surgery, we suggest to use the 0° endoscope, because its usage is more intuitive and immediate. In fact the lens collects images that come before the end of the endoscope. The 30° endoscope is recommended only to a more mature user, experienced in the management of the capture horizon since the correct orientation of the images varies widely with the rotation of the endoscope around its longitudinal axis. In fact, being the loggia mammary similar to a hemisphere, the use of 30° endoscope which eases the vision in the recesses and anfractuosities, it is not so essential.
We try to summarize the characteristics of the endoscopes underlining the advantages of each one.
The advantages of forward looking endoscope (0°) are the following:
most common type of scope, wide choice o market;
effective for most procedures;
higher brightness;
larger field of view;
larger depth of field;
but unable to visualize some operative areas.
The advantages of 30, 50, 70° endoscopes are the following:
possibility to explore areas of the operative field that are difficult to access with a 0° endoscope.
The disadvantages of 30, 50, 70° endoscopes are the following:
lower brightness;
more difficult to control field of view;
light post must be kept vertical to avoid rotating field of view.
Width of View
The width of view of the endoscope usually varies from 20 to 60° although scopes with widths of views of 80° now exist.
Endoscopes are available with diameters from 3 to 12 mm. In general, the narrower the scope the less bright the image and the narrower the width of field. Certain manufacturers have developed endoscopes that are <3 mm in diameter. The principles of function of these endoscopes differ from conventional endoscopes. The image is transmitted via optical fibers, as the rod-lens system is not adapted for such small diameters. The depth of field, optical quality, and light transmitted by these endoscopes are considerably inferior to the standard 10 mm endoscopes. However, the optical quality of these micro-endoscopes is improving, although they remain difficult to use because they are so fragile.
Several manufacturers have developed endoscopes that provide a stereoscopic view of the operating field. Stereoscopic endoscopes transmit two images to a pair of video cameras. The image has to be viewed on a special projection system and a number of different systems exist. The use of stereoscopic systems is currently limited. The only regular use of the stereoscopic vision is for Trans-anal Endoscopic Microsurgery (TEM). They have also been incorporated into robotic laparoscopic systems.
With regard to the video-assisted minimally invasive breast surgery, the choice between endoscopes available is limited to those of 5 and 10 mm in diameter. In principle, the 5 mm endoscopes are efficient enough to provide a perfect vision of the operating field. This depends mainly on the fact that the virtual cavity in which the operation is performed (the mammary loggia), is sufficiently small to be well illuminated with the light produced from the 5 mm endoscope. In this way, we can limit the use of 10 mm endoscope that, although more potent in terms of brightness, are bulker and can create some limitation in the management of the instruments especially in the single port setting. Concerning the robotic platform, the choice does not arise as there is only 8 mm endoscope available. At the moment according to the present optical technology, it is not possible to have optics with two channels for the images acquisition of a smaller diameter.
Flexible and Semi-Flexible Endoscopes
Certain manufacturers have developed systems for surgical endoscopes that are comparable to flexible endoscopes. Two different types of systems exist:
The first has a standard rigid section and a flexible tip. This articulated part consists of a fiber optic bundle just like those found in flexible gastrointestinal endoscopes. The image is otherwise transmitted from the tip to the eyepiece of the endoscope as a light image.
A more innovative system uses a CCD sensor placed at the distal end of the endoscope. Thus, the shaft of the endoscope transmits an electrical signal rather than a light image.
Neither of these two systems are widely used.
All these features define the quality of an endoscope. Often, however, the manufacturers do not report the data for these characteristics and for an average user is still difficult to correctly interpret these data with the expected performance of the instrument. In this case then it is more convenient to rely on a series of small practical devices that allow us to assess the degree of reliability and quality of the endoscope.
So it is possible to check the quality of an endoscope as follows:
“Fish-eye” distortion can be checked by viewing a written text on a piece of paper through the endoscope. If the text looks flat, then the distortion is limited. However, if it looks spherical, the distortion is important.
The depth of field can be assessed by viewing a line of text and analyzing the total near-far distance over which the text appears in focus. This should be over 10 cm. In endoscopes providing a smaller depth of field the surgeon will have to repeatedly readjust the scope’s position.
The brightness of an endoscope is dependent not only upon its light channel and lens system but also upon the light cable and light source. Before assessing the endoscope for light transmission it must be attached to an optimized light source.
Gauge of Endoscope
The wider the endoscope the more light transmitted and thus better the final clarity of the viewed image. Narrower endoscopes have the advantage of passing down smaller trocars but generally have a narrower field of view. Thus a narrow scope may be perfectly adequate for a diagnostic or simple procedure and results in a smaller scar. However, for more complex procedures a broader scope gives a brighter and larger field of view.
An ideal endoscopic surgery kit would include a complete range of endoscopes (0–25, 30, 40, 50, and 70°) and a variety of gauges (12, 10, 5, and even 3 mm). At the present time, since cameras are not sterilized, it is difficult to change endoscopes during the operation while maintaining proper sterilization. Before starting the operation, the surgeon usually has to choose the endoscope that will be used throughout the procedure.
Usage Warning
Most endoscopes have no particular adjustment possibilities. Endoscopes should be used at body temperature or room temperature to prevent too much fog from forming on the end of the endoscope. Warming the scope in a flask of hot saline can be particularly effective. A variety of liquid preparations is available and can be applied to the tip to help reduce fogging.
Endoscopes are designed to be tough in order to protect the fragile systems of lenses that are glued within the scope. If there is an extreme temperature increase or an impact, the orientation of the lenses can be disturbed and the quality of the vision can be altered irreparably. This explains the often short life-span of endoscopes in the operating room.
Before the operation it is important to check the overall condition of the endoscope, search for signs of impact that could have dislodged a lens and caused a distortion of the image or a decrease in the light transmitted.
The light fiber bundle can also be damaged resulting in a decrease in the amount of light transmitted to the operating field.
It is recommended to carefully clean the endoscopes with a plastic brush and soap, especially the end inserted in the abdomen. Indeed, the accumulation of blood and protein at the end of the endoscope, combined with the heat from the light, cause a coagulation of the proteins and a progressive alteration of the quality of the light and image transmitted. For this reason, the tips of endoscopes must be frequently cleaned.
Video Camera
The development and miniaturization of video transmission systems used in videoscopic surgery has made it possible to perform complex endoscopic surgical procedures. The whole surgical team is now able to follow the procedure and participate actively. The images can be recorded on a variety of storage media or teletransmitted to surgeons at a distance. Technological advances mean that it is now possible to obtain excellent image quality, even when operative conditions are difficult (bleeding, emergencies, etc.). The use of high performance cameras should therefore be considered as an essential safety requirement, as well as a means of providing optimal visual comfort for the surgeon.
The quantum leap in the video surgery coincided with the introduction of mini cameras whose technology was directly derived from the NASA experiences in 1980s when, during the space exploration within the Space Shuttle’s program: in fact extremely small and compact video systems have been developed to be inserted into the astronauts suits to assist them in their space walks.
General Description
The camera system is composed of a camera head with its attached cable and a camera control unit.
The camera head attaches to the endoscope and is responsible for capturing the image transmitted along the endoscope and converting it to an electrical signal. The camera control unit receives the signal from the camera head and processes the image in preparation to be sent as a video signal to a monitor. Modern camera heads are about the size of the surgeon’s hand and lightweight to allow for easy manipulation. The camera head construction includes the following important components: (1) the photo CCD; (2) lens and focusing ring (+/− mechanical zoom); (3) coupling mechanism for attaching to endoscope; (4) water-resistant casing and integrated cable.
The Photo CCD
Central to the capture and transmission of the image is the CCD sensor (Charge-Coupled-Device). This is an integrated circuit coated with transparent quartz and comprised of photo sensitive elements (photosites) that transform the captured light image comprised of photons (light energy) into negatively charged electrons (electrical energy). These electrons flow along the camera cable to the camera control box to be processed. When light hits the photosite, an electrical current is generated that is proportional to the intensity of light received. The CCD uses semi-conductor technology and incorporates a grid pattern on which individual photosites, or microscopic photo-sensors, are laid out horizontally and vertically. Each of these photosites generates the signal for a single pixel. (The pixel is the smallest unit on the image) Together the signals from all the photosites create the pattern of pixels that generate the overall image on the screen.
CCD Basic Principle
Resolution
The higher the number of pixels contained in an image, the better the quality will be and thus, greater detail will be contained in the image.
Image Sensor
Image sensors have evolved rapidly as progress has been made in electronics.
At the same time as their capacity to analyze light has increased so they have decreased in size. The size of the CCD is traditionally measured in inches and the ratio of its width to its height is 4:3, corresponding to the shape of video monitors. Initially, image sensors were 2/3 of an inch (8.8 × 6.6 mm), then decreased to 1/2 of an inch (6.4 × 4.8 mm) and more recently to 1/3 of an inch (4.8 × 3.6 mm) or 1/4 of an inch (3.2 × 2.4 mm).
Currently, CCD image sensors offer a resolution ranging from 480 × 320 pixels (153,600 pixels) to several million pixels for the most powerful models. These CCDs are not all adapted for surgical video cameras. The usual resolution of the best surgical cameras is around 450,000 pixels.
Number of CCDs Used
The first video cameras used only one CCD sensor (mono-CCD-analogue) combined with a mosaic filter for the three primary colors (red–green–blue). Such systems required 4 photosites to generate the signal for one pixel (1 red, 2 green, and 1 blue).
Tri CCDs
All new video cameras use a tri-CCD, or 3-chip system (analogue or digital). This incorporates a beam splitter, which divides the light in the image into the three primary colors and directs each to a separate CCD sensor, each sensor being dedicated to one of the primary colors.
Advanced CCD Principle
The information relating to each pixel is converted into binary code (0 or 1) by the analogue–digital converter. There is usually an eight digit binary code (8 bits) per pixel, allowing for 256 different values (28). As each pixel of color is reproduced on the screen by composition of the three primary colors (red, green, and blue), it is possible to create up to 16,777,216 different colors (256 × 256 × 256).
Sensitivity to Light
This parameter, specified in Lux, defines the minimum amount of light needed by the camera to produce a clear image. The lower the number, the less light the camera will need. Ideally, it should be less than 1.5 Lux. The best video cameras can see at <1 Lux/Signal-to-Noise (S/N) ratio:
This measurement, defined in decibels (dB), is directly related to the amplification and the treatment of the video signal by the camera. The higher the ratio, the less risk there will be of interference “noise”, and therefore the purer the signal will be, allowing for a stable and coherent final image. This ratio should not be lower than 50 dB. It is particularly important to compare the S/N ratio for low levels of illumination. Certain cameras, in order to improve their sensitivity to light, have an “image gain” function. This artificial amplification of the video signal causes an increase in interference, which results in a grainier image. It is therefore advisable to compare the sensitivity (Lux) for different cameras that offer an equivalent S/N ratio. Interference is easier to identify in the darker parts of the image. For example, if the signal is ten times higher than the noise, the noise gets lost in the signal and is not noticeable. On the other hand, if the signal is only two times higher, the noise becomes perceptible. This applies to sound in the same way: we notice noise more when we are surrounded by silence!
CCD Structure and Function
There are only a few CCD manufacturers in the world. All manufacturers use the same CCDs, but develop their own signal processing electronics, which represents the main added value of video cameras.
The original CCD used a mechanical shutter to separate the light images reaching the photosites and this technology is now obsolete. Current CCDs are able to continuously receive light without disturbance of image transfer. There are various systems in use today but the most commonly used for videoscopic surgery is called Interline Transfer CCD technology. In this system the photosites are organized into columns and rows on the CCD grid. The first element of the photosite responds to the photons from the image that strikes it by accumulating charge (photoelectric effect). This packet of electrical charge is proportional to the quantity of photons received during a given time (integration time). This packet of charge is transferred into a storage and transfer channel called the vertical shift register. The packets of charge are then transferred to a single horizontal shift register, which lies beneath the vertical register. All the packets from the horizontal register are then sent to a memory buffer before they are amplified and sent to an analogue–digital converter. This sequence occurs every 1/50th of a second with a PAL system (1/60th of a second with NTSC), alternating the odd and even lines, in order to be compatible with the various available video display systems (two interlaced fields—see chapter on video monitors).
CCD Sensor with Micro Lenses
In order to recuperate the “lost” photons between each photosite on the CCD sensor, micro-lenses are etched on each of these photosites to direct and concentrate all of the photons onto the photosensitive cells. This improves the camera’s sensitivity to light, without digital signal treatment.
Lens and Focus Ring
Before the image from the endoscope reaches the photo CCD, it is focused by a lens placed in front of the CCD. A focus ring allows the surgeon to make adjustments to the distance between the lens and the CCD so that the focus of the observed object in the image can be kept sharp. The newest systems have an autofocus mechanism that controls the lens in response to changes in the observed image. If the camera is fitted with a mechanical zoom the focal length of the camera can be adjusted. By moving the CCD and focusing lens away from the eyepiece lens of the endoscope the image can be magnified, however, the field of view reduced. The majority of camera systems utilize a digital zoom, which means that the image processor in the camera control unit simply magnifies each pixel. Thus although the image may appear magnified the resolution is diminished, leading to a decrease in the quality of the image, which becomes more apparent the more the zoom is used.
The Coupling Mechanism
The camera head needs to be securely coupled to the endoscope to ensure accurate transmission of the image. The eyepiece of most endoscopes is fairly standard but the coupling devices for each manufacturer tend to be different. The coupling device holds the camera lens at a fixed distance from the eyepiece lens of the endoscope. Manufacturers design their cameras to match exactly with their endoscopes; for this reason mixing different systems can sometimes lead to loss of image quality.
Camera Casing and Cable
The camera casing is designed to protect the encased electronics and lens system. It needs to be able to withstand cleaning procedures and a degree of mechanical stress. Special alloys and plastics are used to construct the tough, resistant casing. Frequent usage inevitably leads to deterioration in the surface of the casing. However, significant damage to the casing should be repaired to avoid possible damage to the delicate electronics contained within.
Camera Input
The camera is connected to the camera control unit via a shielded cable. Each manufacturer has a unique multipin socket, usually placed on the front of the camera control unit to which this cable connects. The camera plug is supplied with a watertight cap to prevent water getting into the electrical connections during the cleaning process.
Video Signal Outputs
Located, usually, on the back of the camera control unit are the connections for the video monitor. Because there are a variety of industry standards for video signal transfer, the manufacturers have provided a selection of possible connections on the back of the camera control unit.
Connection 1
RGB (red–green–blue): The video signal for each color is carried by a separate shielded cable and synchronized with a fourth cable (RGB-S). Brightness (Y) is calculated by adding the three colors according to the formula shown above. Four separate BNC (Bayonet Neill Concelman) connections are used.
Connection 2
Y/C or S-video = S-VHS:
This carries two separate signals, one for light intensity and synchronization (Y), and one for chrominance (C). Using the formula above there is sufficient information in this single signal to calculate levels of each of the primary colors. The cable is usually shielded and carries four wires (+/− for each signal). A four pin connection is used (some manufacturers use a 6 pin socket and carry additional control signals on the additional cables).
Composite Video
This carries a single analogue signal along a shielded cable. The signal for light intensity and color (chrominance) are combined into a single signal. A BNC connection is used.
Digital
Digital output connection (e.g., DVI): this type of connection is not widely used, because it must be connected to a LCD or computer monitor. Presently, the display quality is inferior to a classic cathode ray tube monitor. This connection permits recording on a digital medium without deterioration of the signal.
The quality of the transmitted image is dependant on the type of connection used. From an electronic point of view, the more information sent from the camera control unit to the monitor per unit time the better the potential quality of the image. Thus in theory, in an otherwise calibrated and optimized system the lowest quality is obtained by the single cable composite BNC connection and the optimal quality is obtained by the RGB connection. However, since the resolution is generally limited by the monitor, there is no notable improvement with a RGB connection as compared to a Y/C connection. It should be noted that with a RGB connection, the colors cannot be modified by the controls on the monitor.